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Oligosaccharides & Sugar nucleotides

Kyowa Hakko Bio (KHB) established a novel production system for oligosaccharides and sugar nucleotides. Oligosaccharides are expected as potential neutraceuticals and sugar nucleotides are used for enzymatic synthesis of oligosaccharides.

Artificial Biosynthesis of Sugar nucleotides (ABS system)

Sugar nucleotide production system was established by the combination of recombinant Escherichia coli expressing the genes involved in sugar nucleotide biosynthesis and Corynebacterium ammoniagenes that has a strong activity to form nucleoside 5 Etriphosohate such as UTP and GTP.
We confirmed the production of
GDP-fucose, GDP-mannose,
CMP-acetylneuraminic acid, UDP-glucose,
UDP-galactose and UDP-N-acetylglucosamine.

Artificial Biosynthesis of Carbohydrate (ABC system)

Oligosacchride production system was established by the coupling of recombinant Escherichia coli expressing the gene of glycosyltransferase with the sugar nucleotide production system. We confirmed the production of oligosaccharides such as 3'-sialyllactose, 6'-sialyllactose, 2'-fucosyllactose, globotriose, lacto-N-tetraose, lacto-N-neotetraose.

Reference

  1. S. Koizumi, T. Endo, K. Tabata, A. Ozaki, Large-scale production of UDP-galactose and globotriose by coupling metabolically engineered bacteria. Nat. Biotechnol., 16, 847-850 (1998)
  2. T. Endo, S. Koizumi, K. Tabata, S. Kakita, A. Ozaki, Large-scale production of N-acetyllactosamine through bacterial coupling., Carbohydr. Res., 316, 179-183 (1999)
  3. T. Endo, S. Koizumi, K. Tabata, A. Ozaki, Large-scale production of CMP-NeuAc and sialylated oligosaccharides through bacterial coupling., Appl. Microbil. Biotechnol., 53, 257-261 (2000)
  4. K. Tabata, S. Koizumi, T. Endo, A. Ozaki, Production of UDP-N-acetylglucosamine by coupling metabolically engineered bacteria. Biotechnol. Lett., 22, 479-483 (2000)
  5. T. Endo, S. Koizumi, K. Tabata, A. Ozaki, Cloning and expression of beta 1,4-galactosyltransferase gene from Helicobacter pylori. Glycobiology, 10, 809-813 (2000)
  6. T. Endo, S. Koizumi, Large-scale production of oligosaccharides using engineered bacteria. Curr. Opin. Struct. Biol., 10, 536-541 (2000)
  7. S. Koizumi, T. Endo, K. Tabata, H. Nagano, J. Ohnishi, A. Ozaki, Large-scale production of GDP-fucose and Lewis X by bacterial coupling., J. Ind. Microbiol. Biotechnol., 25, 213-217 (2000)
  8. T. Endo, S. Koizumi, K. Tabata, Shingo kakita, A. Ozaki, Large-scale production of carbohydrate portion of the sialyl-Tn epitope through bacterial coupling., Carbohydr.Res., 330, 439-443 (2001)
  9. K. Tabata, S. Koizumi, T. Endo, A. Ozaki, Production of N-acetyl-D-neuraminic acid by coupling bacteria expressing N-acetyl-D-glucosamine 2-epimerase and N-acetyl-D-neuraminic acid synthetase., Enzyme Microbial Technol., 30, 327-333 (2002)
  10. S. Koizumi,Production of oligosaccharides by conjugate of biosyntheses system. Bioscience and industry,60, 23-26 (2002)
  11. S.Koizumi,A large amount of synthesis of oligosaccharide by microorganism.Bioscience and industry,20, 39-43 (2003)
  12. S. Koizumi, Large-scale production of oligosaccharides using bacterial functions. Trends in Glycosci. Glycotech., 15, 65-74 (2003)
  13. S. Koizumi, Production of oligosaccharides by coupling engineered bacteria., pp153-164 in Synthesis of Carbohydrate through Biotechnology (ed. P. G. Wang and Y. Ichikawa), Oxford University Press (2004)
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