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R&D

Technical Research Laboratory

2016
  • Takeno S, Hori K, Ohtani S, Mimura A, Mitsuhashi S, Ikeda M. l-Lysine production independent of the oxidative pentose phosphate pathway by Corynebacterium glutamicum with the Streptococcus mutans gapN gene. Metab Eng. 2016 Sep;37:1-10.
  • Mizuno Y, Nagano-Shoji M, Kubo S, Kawamura Y, Yoshida A, Kawasaki H, Nishiyama M, Yoshida M, Kosono S. Altered acetylation and succinylation profiles in Corynebacterium glutamicum in response to conditions inducing glutamate overproduction. Microbiologyopen. 2016 Feb; 5(1): 152–173.
2015
  • Koketsu K, Shomura Y, Moriwaki K, Hayashi M, Mitsuhashi S, Hara R, Kino K, Higuchi Y. Refined regio- and stereoselective hydroxylation of L-pipecolic acid by protein engineering of L-proline cis-4-hydroxylase based on the X-ray crystal structure. ACS Synth Biol. 2015;4(4):383-92.
  • Ikeda M, Noguchi N, Ohshita M, Senoo A, Mitsuhashi S, Takeno S. A third glucose uptake bypass in Corynebacterium glutamicum ATCC 31833. Appl Microbiol Biotechnol. 2015;99(6):2741-50.
  • Nakajima S, Satoh Y, Yanashima K, Matsui T, Dairi T. Ergothioneine protects Streptomyces coelicolor A3(2) from oxidative stresses. J Biosci Bioeng. 2015: S1389-1723(15)00034-1.
2014
  • Ujihara T, Nagano M, Wada H, Mitsuhashi S. Identification of a novel type of polyunsaturated fatty acid synthase involved in arachidonic acid biosynthesis. FEBS Lett. 2014;588(21):4032-6.
  • Shimizu T, Yoshiura H, Nagano H, Hirasawa I. Effect of Specific Amino Acids on Controlling Crystal Pseudopolymorphism of L-Arginine Hydrochloride. Chemical Engineering & Technology. 2014; 37(8): 1427–1430.
  • Mitsuhashi S. Current topics in the biotechnological production of essential amino acids, functional amino acids, and dipeptides. Curr Opin Biotechnol. 2014;26:38-44.
  • Satoh Y, Kuratsu M, Kobayashi D, Dairi T. New gene responsible for para-aminobenzoate biosynthesis. J Biosci Bioeng. 2014;117(2):178-83.
2013
  • Yoshiura H, Nagano H, Hirasawa I. New Insights into Additive Structure Effect on Crystal Agglomeration of L-Valine. Chemical Engineering & Technology. 2013; 36(12): 2023–2028.
  • Yoshiura H, Nagano H, Hirasawa I. Mechanism of specific influence of l-Glutamic acid on the shape of l-Valine crystals. J Crystal Growth. 2013; 363(15): 55-60
  • Takeno S, Takasaki M, Urabayashi A, Mimura A, Muramatsu T, Mitsuhashi S, Ikeda M. Development of fatty acid-producing Corynebacterium glutamicum strains. Appl Environ Microbiol. 2013;79(21):6776-83.
  • Ikeda M, Miyamoto A, Mutoh S, Kitano Y, Tajima M, Shirakura D, Takasaki M, Mitsuhashi S, Takeno S. Development of Biotin-Prototrophic and -Hyperauxotrophic Corynebacterium glutamicum strains. Appl Environ Microbiol. 2013;79(15):4586-94.
  • Hayashi M, Tabata K.Metabolic engineering for L-glutamine overproduction by using DNA gyrase mutations in Escherichia coli.Appl Environ Microbiol. 2013 ;79(9):3033-9.
  • Koketsu K, Mitsuhashi S, Tabata K. Identification of homophenylalanine biosynthetic genes from the cyanobacterium Nostoc punctiforme PCC73102 and application to its microbial production by Escherichia coli. Appl Environ Microbiol. 2013 ;79(7):2201-8.
  • Takeno S, Shirakura D, Tsukamoto N, Mitsuhashi S, Ikeda M. Significance of the Cgl1427 gene encoding cytidylate kinase in microaerobic growth of Corynebacterium glutamicum. Appl Microbiol Biotechnol. 2013 ;97(3):1259-67.
2012
  • Suzuki M, Takahashi Y, Noguchi A, Arai T, Yagasaki M, Kino K, Saito J.The structure of L-amino-acid ligase from Bacillus licheniformis.Acta Crystallogr D Biol Crystallogr. 2012 ;68(Pt 11):1535-40.
  • Sakihama Y, Mizoguchi H, Oshima T, Ogasawara N. YdfH identified as a repressor of rspA by the use of reduced genome Escherichia coli MGF-01. Biosci Biotechnol Biochem. 2012;76(9):1688-93.
  • Shomura Y, Hinokuchi E, Ikeda H, Senoo A, Takahashi Y, Saito J, Komori H, Shibata N, Yonetani Y, Higuchi Y. Structural and enzymatic characterization of BacD, an L-amino acid dipeptide ligase from Bacillus subtilis. Protein Sci. 2012;21:707-16.
2011
  • Ikeda M, Mizuno Y・Ikeda M, Mizuno Y, Awane S, Hayashi M, Mitsuhashi S. & Takeno S. Identification and application of a different glucose uptake system that functions as an alternative to the phosphotransferase system in Corynebacterium glutamicum. Appl Microbiol Biotechnol. 2011;90:1443-1451.
  • Arakawa C, Kuratsu M, Furihata K, Hiratsuka T, Itoh N, Seto H, and Dairi T, Diversity of the Early Step of the Futalosine Pathway. Antimicrob Agents Chemother. 2011;55:913-916.
  • Awane S, Hayashi M, Mitsuhashi S. & Takeno S. Identification and application of a different glucose uptake system that functions as an alternative to the phosphotransferase system in Corynebacterium glutamicum. Appl Microbiol Biotechnol. 2011;90:1443-1451.
2010
  • Kuratsu M, Hamano Y, and Dairi T. Analysis of the metabolic pathway in Lactobacillus. Appl Environ Microbiol. 2010;76:7299-7301.
  • Takeno S, Murata R, Kobayashi R, Mitsuhashi S. & Ikeda M. Engineering of Corynebacterium glutamicum with an NADPH-generating glycolytic pathway for L-lysine production. Appl Environ Microbiol. 2010;76:7154-7160
  • Senoo A, Tabata K, Yonetani Y, Yagasaki M. Identification of novel L-amino acid alpha-ligases through Hidden Markov Model-based profile analysis. Biosci Biotechnol Biochem. 2010;74:415-418.
  • Ishikawa M, Koizumi S. Microbial production of N-acetylneuraminic acid by genetically engineered Escherichia coli. Carbohydr Res. 2010;345:2605-2609.
  • Hayashi M, Tabata K, Yagasaki M, Yonetani Y. Effect of multidrug-efflux transporter genes on dipeptide resistance and overproduction in Escherichia coli. FEMS Microbiol Lett. 2010;304:12-19.
2009
  • Ikeda M, Baba M, Tsukamoto N, Komatsu T, Mitsuhashi S. & Takeno S. Elucidation of genes relevant to the microaerobic growth of Corynebacterium glutamicum. Biosci Biotechnol Biochem 2009;73:2806-2808.
  • Gondry M, Sauguet L, Belin P, Thai R, Amouroux R, Tellier C, Tuphile K, Jacquet M, Braud S, Courcon M, Masson C, Dubois S, Lautru S, Lecoq A, Hashimoto S, Genet R, Pernodet JL.　Cyclodipeptide synthases are a family of tRNA-dependent peptide bond-forming enzymes. Nature Chemical Biology. 2009;5:414-420.
  • Ikeda M, Mitsuhashi S, Tanaka K, Hayashi M. Reengineering of a Corynebacterium glutamicum L-arginine and L-citrulline producer. Appl Environ Microbiol. 2009;75:1635-41.
  • Kino K, Kotanaka Y, Arai T, Yagasaki M: A novel L-amino acid ligase from Bacillus subtilis NBRC3134, a microorganism producing peptide-antibiotic rhizocticin. Biosci Biotechnol Biochem 2009; 73:901-907.
  • Miyake K, Kakita S. A novel catalytic ability of γｰglutamylcysteine synthetase of Escherichia coli and its application for theanine production. Biosci Biotech Biochem. 2009;73:2677-2683.
2008
  • Ohnishi J, Mizoguchi H, Takeno S, Ikeda M. Characterization of mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine in an industrial Corynebacterium glutamicum strain. Mutat Res. 2008;649:239-44.
  • Takeno S, Nakamura M, Fukai R, Ohnishi J, Ikeda M. The Cgl1281-encoding putative transporter of the cation diffusion facilitator family is responsible for alkali-tolerance in Corynebacterium glutamicum. Arch Microbiol. 2008;190:531-538.
  • Kino K, Noguchi A, Nakazawa Y, Yagasaki M: A novel l-amino acid ligase from Bacillus licheniformis. J Biosci Bioeng 2008; 106:313-315.
  • Kino K, Nakazawa Y, Yagasaki M: Dipeptide synthesis by L-amino acid ligase from Ralstonia solanacearum. Biochem Biophys Res Commun 2008; 371:536-540.
  • Hashimoto S. Ribosome-independent peptide synthesis in nature and their application to dipeptide production.　J Biol Macromol. 2008;8:28-37.
  • Yagasaki M, Hashimoto S. Synthesis and Application of Dipeptides; Current Status and Perspectives. Appl Microbiol Biotechnol. 2008;81:13-22.
2007
  • Tabata K, Hashimoto S. Fermentative production of L-alanyl-L-glutamine by a metabolically engineered Escherichia coli strain expressing L-amino acid alpha-ligase. Appl Environ Microbiol. 2007;73(20):6378-85.
  • Takeno S, Ohnishi J, Komatsu T, Masaki T, Sen K, Ikeda M. Anaerobic growth and potential for amino acid production by nitrate respiration in Corynebacterium glutamicum. Appl Microbiol Biotechnol. 2007;75:1173-1182.
2006
  • Hayashi M, Ohnishi J, Mitsuhashi S, Yonetani Y, Hashimoto S, Ikeda M. Transcriptome analysis reveals global expression changes in an industrial L-lysine producer of Corynebacterium glutamicum. Biosci Biotechnol Biochem. 2006;70(2):546-50.
  • Ohnishi J, Ikeda M. Comparisons of potentials for L-lysine production among different Corynebacterium glutamicum strains. Biosci Biotechnol Biochem. 2006 ;70(4):1017-20.
  • Ikeda M, Ohnishi J, Hayashi M, Mitsuhashi S. A genome-based approach to create a minimally mutated Corynebacterium glutamicum strain for efficient L-lysine production. J Ind Microbiol Biotechnol. 2006;3(7):610-5.
  • Hayashi M, Mizoguchi H, Ohnishi J, Mitsuhashi S, Yonetani Y, Hashimoto S, Ikeda M. A leuC mutation leading to increased L-lysine production and rel-independent global expression changes in Corynebacterium glutamicum.　Appl Microbiol Biotechnol. 2006;72(4):783-9.
  • Mitsuhashi S, Hayashi M, Ohnishi J, Ikeda M. Disruption of malate:quinone oxidoreductase increases L-lysine production by Corynebacterium glutamicum. Biosci Biotechnol Biochem. 2006; 70(11):2803-6.

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